親愛的使用者 您好：

以下為藉此次農業部查核，將腫瘤通報流程完整建立，也避免user後續困擾。

 

一、腫瘤之異常通報

(1) A區

為種原庫，正常來說腫瘤發生機率較小(除非年紀或表現型)。

因此一旦有腫瘤(飼育員/獸醫師)就會開立異常提醒(正式)，目的：提醒user留意尺寸大小；

並後續以卡尺測量任一腫瘤直徑接近或超過2cm或表面明顯潰瘍/壞死時，由獸醫師依照SOP指示user犧牲。

 

 

 

(2) B區/C區

 

 

 

注射腫瘤進行實驗，性質上屬於預期之異常現象，

 

 

 

當以卡尺測量任一腫瘤直徑>1.5cm(飼育員/獸醫師)就會開立異常提醒(正式)，目的：提醒user留意尺寸大小；

 

 

 

並後續以卡尺測量任一腫瘤直徑接近或超過2cm或表面明顯潰瘍/壞死時，由獸醫師依照SOP指示user犧牲。

 

 

 

 

 

 

 

二、腫瘤人道處理SOP之判定

 

 

 

依據院方IACUC及農業部<實驗動物準則>規範

 

 

 

- 腫瘤單邊直徑在小鼠超過 2 公分 、在大鼠超過 4 公分。

 

 

 

- 腫瘤轉移或快速增長至表面出現感染或潰瘍/壞死 ，或是腫瘤已致使其它臟器功能出現明顯異常時。

 

 

 

- 腫瘤致使小鼠體重降低20%以上或BCS為1或2。

 

 

 

- 影響進食、飲水(無法行動、無法抬頭)。

 

 

 

 

 

 

 

 

 

 

Dear LAB user,

 

 

 

 

 

 

 

1. Abnormality Reporting SOPs of Tumor case

 

 

 

(1) Area A

 

 

 

For breeding purpose, the chance of tumor occurrence is normally low (unless of age or phenotype).

 

 

 

Therefore, if there is a tumor (our caretaker/veterinarian), an "Abnormality" will be informed. To remind the user of paying attention to the tumor size.

 

 

 

If any diameter of tumor measured with a caliper approaches or exceeds 2 cm or any obvious ulcer/necrosis on the surface, the veterinarian will suggest the user to sacrifice the animal according to the SOP.

 

 

 

(2) Area B/Area C

 

 

 

The tumor inoculation experiment was an expected abnormal phenomenon.

 

 

 

When the tumor is measured with a caliper and any diameter is >1.5cm (our caretaker/veterinarian), an "Abnormality" will be informed. To remind the user of paying attention to the tumor size.

 

 

 

If any diameter of tumor measured with a caliper approaches or exceeds 2 cm or any obvious ulcer/necrosis on the surface, the veterinarian will suggest the user to sacrifice the animal according to the SOP.

 

 

 

 

 

 

 

2. Determination for Humane Treatment of Tumor case

 

 

 

According to the regulations of IACUC and the <Guideline for Experimental Animals> of Ministry of Agriculture
- The diameter of a single side of the tumor exceeds 2 cm in mice and 4 cm in rats.
- When the tumor metastasizes or grows rapidly to the point where infection or necrosis/ulceration occurs on the surface, or when the tumor has caused obvious abnormalities in the functions of other organs.
- The tumor caused the mice to lose more than 20% of their body weight or have a BCS of 1 or 2.
- Affects eating and drinking (unable to move, unable to raise head).