一、以下事項之實驗動物，必須申請進入B1房(手術後恢復室)及手術操作區：
(一) 施打腫瘤細胞。
(二) 欲執行「手術」。
PS.手術的定義為開顱/開胸/開腹等…切開體腔其中一部分並後續縫合或釘合的手術。因此單純的皮下注射/腹腔注射/靜脈注射等並不算是「手術」。
二、手術前務必先確認「麻醉深度」，以「後腳掌疼痛反射(pinch reflect test)」作為黃金指標。
三、剃毛區也應該在手術區「外」進行、在分開之區域剃毛並清除散落毛髮後，再將動物移至手術區。手術剃毛範圍應至少超過預定下刀的切口兩側各約1公分，避免術區被毛髮汙染。
四、麻醉後建議塗抹眼藥膏，因為動物會喪失眨眼反射、須保護角膜避免乾燥產生感染、產生實驗變項。
五、術後建議採用滅菌擦手紙覆蓋底部，取代直接躺臥於墊料上，動物請「側躺」勿仰躺(避免吸入性肺炎)，視情形於鼠籠外使用保溫墊或加熱墊維持術後動物體溫。術後請持續觀察到至少確認動物已甦醒，人員才可以離開，避免術後死亡或被同伴啃食的風險。
六、請觀察動物術後之疼痛情況，未適時舒緩疼痛會讓動物減少活動力，也可能導致動物減少攝食及飲水的慾望，並阻礙手術部位的復原，故手術後止痛藥(甚至術前先制給予，能產生較長效果)、抗生素、抗發炎藥及其它藥物需要持續給予。
七、手術後的動物觀察應至少3天，並登記於公動手術室內的「術後觀察紀錄」。
八、術後7-14天就應移除動物皮膚上的縫線、皮釘、釘針(除非經IACUC計畫通過可延長拆除時機，或經獸醫師評估動物復原狀況後予以核可)。
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1. Requirements for animal be introduced into Room B1 (Recovery Room) and Operating Room:
(1) Injection of tumor cell.
(2) Surgery.
PS. Surgery is defined as any procedure involving opening a portion of the body cavity, such as a craniotomy, thoracotomy, or laparotomy, followed by suturing. Therefore, simply subcutaneous, intraperitoneal, or intravenous injection do not be counted as a surgery.
2. Before surgery, it is essential to confirm the “depth of anesthesia,” using the “pinch reflect test on the hind foot" as the gold standard.
3. The shaving procedure should also be performed "outside" the surgical area. After shaving and removing loose hair in the separated area, the animal be moved to the surgical area. The shaving area should extend at least 1 cm beyond each side of the planned incision site to avoid contamination of the surgical area with hair.
4. It is recommended to apply eye ointment after anesthesia, as animal may lose their blinking reflex, the cornea must be protected from dryness leading to infection, and experimental variables may arise.
5. It is recommended to cover the floor with autoclaved tissue instead of laying directly on bedding post-surgery. Animal should lie on their sides, not on their backs, to prevent aspiration pneumonia. A warming pad or heating pad should be used outside the cage to maintain its body temperature. After surgery, please observe the animal until it is fully awake before leaving to avoid the risk of postoperative death or being bitten.
6. Observe the animal's pain after surgery. Failure to provide pain relief can reduce activity and animal’s appetite, thus hindering recovery. Therefore, painkillers, antibiotics, anti-inflammatory drugs, and other medications should be administered continuously after (or even before to provide the drug duration) surgery.
7. Post-operative animal observation should last at least 3 days and should be recorded in the "Post-surgery Observation Record" in our operating room.
8. Sutures and staples should be removed from the animal's skin 7-14 days after surgery (unless approved by IACUC or by the veterinarian after evaluating the animal's recovery).